HiYield-T7 Cell-free E. coli Lysate

About this item

Our E. coli cell-free lysates are the isolated protein purification machinery of bacterial cells. They create a high-yielding and adjustable cell-free protein expression system.

Origin of MSP protein: Rat
Purity: >90% (Determined by SDS-PAGE)
Number of amino acids: 210
Molecular mass: >24614 Da
Extinction coefficient (in water) ε280: 33460 M⁻¹ cm⁻¹
Buffer: 20 mM TRIS pH 7.4, 100 mM NaCl, 0.5 mM EDTA
Affinity tag: N-Terminal His-tag

The advantages of cell-free protein expression are numerous, among other things it enables a scientist to:
- For direct stabilization and co-translational integration of membrane proteins into nanodiscs
- Expression of otherwise toxic proteins

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