Nanodisc Mouse MSP2N2-His DMPG

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

• Origin of the MSP protein: Mouse
• Purity: >90% (Determined by SDS-PAGE)
• Number of amino acids: 388
• Molecular Mass: 45526 Da
• Extinction coefficient (in water) ε280: 39880 M⁻¹cm⁻¹
• Buffer: 20 mM TRIS pH 7.4, 100 mM NaCl, 0.5 mM EDTA
• Affinity tag: N-Terminal His-tag

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP2N2 (mouse) creates nanodiscs of ∼17 nm in diameter. This product contains a His-tag, keep that in mind when choosing the affinity tag for your membrane protein. There are multiple phospholipids to choose from that can make up the body of the nanodisc. Which is best, depends on your protein.