The protein purification with the PureCube Glutathione Agarose and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.

• Usage: Specific binding and purification of GST-tagged proteins
• Specificity: Affinity to GST(Glutathione-S-Transferase)-tagged proteins
• Binding capacity: >10 mg/ml
• Bead Ligand: L-Glutathione (reduced)
• Bead size: 40 μm
• Matrix: 7.5% cross-linked agarose
• Filling quantity: Delivered as a 50% suspension
• pH Stability: 3 to 12
• Required equipment:
• Lysis Buffer
• Wash Buffer
• Elution Buffer
• Ice bath
• Refrigerated centrifuge for 50 ml tube (min 10000×g)
• 50 ml centrifuge tube
• Micropipettor and Micropipetting tips
• Disposable gravity flow columns with capped bottom outlet, 2 ml
• pH meter
• End-over-end shaker
• SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

The pairing of an enzyme to its substrate is one of the most specific combinations in life sciences. PureCube Glutathione Agarose is a suspension of tiny 40 µm purification beads that are used for this purpose. Their small size provides high mechanical stability. They can be used for all sorts of affinity chromatography methods, such as FPLC, batch spin, or column chromatography. You can also purchase these agarose beads pre-packed into FPLC-ready cartridges. Additionally, we offer GST-tag affinity resin with a bead diameter of 400 µm for very viscous cell media and higher FPLC flow rates. Alternatively, Glutathione MagBeads are also available.