Our PureCube Co-NTA agarose resins are tiny agarose resin beads with a diameter of 40 µm. They are used for the purification of active His-tagged proteins from cells.

• Usage: Specific binding and purification of 6× His tagged proteins
• Specificity: Affinity to His tagged proteins
• Binding capacity: >30 mg/ml
• Bead Ligand: Co-NTA
• Bead size: 40 μm
• Filling quantity: Delivered as a 50% suspension
• Required equipment:
• Lysis Buffer
• Wash Buffer
• Elution Buffer
• Ice bath
• Refrigerated centrifuge for 50 ml tube (min 10000×g)
• 50 ml centrifuge tube
• Micropipettor and Micropipetting tips
• Disposable gravity flow columns with capped bottom outlet, 2 ml
• pH meter
• End-over-end shaker
• SDS-PAGE buffers, reagents and equipment Optional: Western -Blot reagents and equipment

Our year-long experience in manufacturing agarose resin led to a high yield of 40 mg protein per ml resin, leading in the market compared to other Co-NTA suppliers. PureCube Co-NTA resins are suited for batch spin columns and FPLC. Their small diameter provides them with excellent mechanical stability. We recommend our Co-NTA agarose beads with 100 µm diameter for higher flow rates and, for extreme cases, XL-sized Co-NTA beads. Furthermore, these beads are also available pre-packed in a column/cartridge or as magnetic beads.