Human Recombinant IL-12 (IL12A and IL12B Heterodimer) Protein (from HEK293 Cells)

About this item

A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (P29459.2) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

Shipping temperature: Dry ice

Specifications

Conjugation:
Unconjugated
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Endotoxin Content:
<5 EU per mg of the protein
Biological Activity:
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.04 - 0.4 ng/ml. The specific activity of recombinant human IL-12 is >10,000 Units/μg, which is calibrated against the human IL-12 WHO International Standard (NIBSC code: 95/544).
Endotoxin-free:
No
Carrier-Free:
Yes
Animal-Free:
No
Protein Synonyms:
IL12 p70|NKSF|IL12|IMD29|CLMF|CLMF2|NKSF2|IL-12A|IL-12B|IMD28|P35|NFSK|NKSF1
Protein/Peptide Name:
IL12A & IL12B
Purity:
≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.