Human TRPC5 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human TRPC5 in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Detection range: 0.75 to 12 ng/ml
• Sensitivity: 0.032 ng/ml
• Sample type: Serum, plasma or other biological fluids
• Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Human TRPC5 ELISA kit (A310279) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human TRPC5 in An antibody specific for TRPC5 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TRPC5 present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-TRPC5 antibody, which also binds the TRPC5 present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-TRPC5 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-TRPC5 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of TRPC5 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of TRPC5 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.