Human P4HB ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human P4HB in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Detection range: 1.5 to 24 ng/ml
• Sensitivity: 0.055 ng/ml
• Sample type: Serum, plasma or other biological fluids
• Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Human P4HB ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human P4HB in serum, plasma or other biological fluids. An antibody specific for P4HB has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the P4HB present in each sample is bound to the wells by the immobilized antibody. Biotinylated Anti-P4HB antibody, which also binds the P4HB present in each sample, and Streptavidin-HRP, which binds the Biotinylated Anti-P4HB antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated Anti-P4HB antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of P4HB captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of P4HB in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.