Mouse CD134/OX40L receptor ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse CD134/OX40L receptor in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Mouse CD134/OX40L receptor ELISA kit (A313223) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse CD134/OX40L receptor in serum, plasma or other biological fluids. An antibody specific for CD134/OX40L receptor has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CD134/OX40L receptor present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-CD134/OX40L receptor antibody, which also binds the CD134/OX40L receptor present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-CD134/OX40L receptor antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-CD134/OX40L receptor antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of CD134/OX40L receptor captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of CD134/OX40L receptor in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.