Mouse As3mt ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse As3mt in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutes, with a single wash step
• Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Mouse As3mt ELISA kit (A312150) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse As3mt in serum, plasma or other biological fluids. An antibody specific for As3mt has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the As3mt present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-As3mt antibody, which also binds the As3mt present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-As3mt antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-As3mt antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of As3mt captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of As3mt in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.