The Ph.D.-C7C Phage display peptide library kit v2 is based on a combinatorial library of random disulfide looped peptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage.

• Ready to use complex phage library (~1E09 clones)
• Structurally constrained peptide library
• Panning against Mouse mAb yields more reliable epitope results than former target protein
• Does not require helper phage for amplification
• Inherent link between phenotype and genotype allows screening of billions of clones in a single tube

The library is displayed in the form AC-X7-C-GGG, which directly precedes the wild-type pIII sequence. Under neutral conditions disulfide loop formation is favored. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 μl of the supplied phage. The Ph.D.-C7C Phage display peptide library kit v2 contains the Ph.D.-C7C Phage display peptide library, a DYKDDDDK Mouse monoclonal antibody and Protein G Magnetic beads for a panning control experiment, and enough -96 g III sequencing primer for >50 sequencing reactions.