Isolate up to 25 µg plasmid DNA from 1 to 5 ml bacterial cultures using mini spin columns.

• Rapid - Purification of plasmid DNA in less than 30 minutes
• Safe - No Phenol/chloroform extractions
• Versatile - Spin and vacuum formats available
• High-quality - DNA is suitable for a variety of downstream applications

The E.Z.N.A.® Plasmid DNA Mini Kit I is designed to isolate up to 25 µg of high-quality plasmid DNA from 1 to 5ml bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with HiBind® Mini Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.