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Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Human
- Target protein:Tartrate Resistant Acid Phosphatase
- Description:Human tartrate resistant acid phosphatase ELISA kit
- Size:96 tests
- Environmentally Preferable:
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:Human Tartrate Resistant Acid Phosphatase ELISA Kit exhibits high specificity and excellent specificity for the detection of human Tartrate Resistant Acid Phosphatase. No significant cross-reactivity or interference between Tartrate Resistant Acid Phosphatase and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:0.313 - 20 mIU/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:0.188 mIU/ml
- Regulatory Status:RUO
- Cat. No.:76742-382
Specifications
About this item
Human Tartrate Resistant Acid Phosphatase ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Tartrate Resistant Acid Phosphatase in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 0.313 to 20 mIU/ml
- Sensitivity: 0.188 mIU/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Human Tartrate Resistant Acid Phosphatase ELISA Kit (A74480) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Tartrate Resistant Acid Phosphatase in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Tartrate Resistant Acid Phosphatase has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Tartrate Resistant Acid Phosphatase present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Tartrate Resistant Acid Phosphatase Antibody, which binds the captured Tartrate Resistant Acid Phosphatase present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Tartrate Resistant Acid Phosphatase captured in each well. The concentration of Tartrate Resistant Acid Phosphatase can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.