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Human uPA ELISA Kit
Human uPA ELISA Kit
  76741-952
 :  ANTIBODIES.COM LLC
 :  
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Human uPA ELISA Kit
  76741-952
 :  ANTIBODIES.COM LLC
 :  A74572-96
 :  

 

  • Assay duration:
    Multiple steps
  • Assay Type:
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Human
  • Target protein:
    uPA
  • Description:
    Human uPA ELISA kit
  • Size:
    96 tests
  • Sample Type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross Reactivity:
    Human uPA ELISA kit exhibits high specificity and excellent specificity for the detection of human uPA. No significant cross-reactivity or interference between uPA and analogues was observed.
  • Detection Method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf Life:
    Store for 6 months at 4 °C
  • Detection Range:
    62.5 - 4000 pg/ml
  • Storage Temperature:
    4 °C
  • Sample Volume:
    100 μl
  • Sensitivity:
    37.5 pg/ml
  • Regulatory Status:
    RUO
  • Cat. No.:
    76741-952

 

 

Human uPA ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human uPA in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready-To-Use ELISA Kit
  • Detection Range: 62.5 - 4000 pg/ml
  • Sensitivity: 37.5 pg/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%

Human uPA ELISA kit (A74572) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human uPA in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for uPA has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the uPA present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-uPA Antibody, which binds the captured uPA present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of uPA captured in each well. The concentration of uPA can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

 : This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilized standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin Conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.