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Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:Laminin gamma 3
- Description:Mouse laminin gamma 3 ELISA kit
- Size:96 tests
- Sample Type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross Reactivity:Mouse Laminin gamma 3 ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse Laminin gamma 3. No significant cross-reactivity or interference between Laminin gamma 3 and analogues was observed.
- Detection Method:Colorimetric
- Time to Results:4 h 30 min
- Shelf Life:Store for 6 months at 4 °C
- Detection Range:15.625 - 1000 pg/ml
- Storage Temperature:4 °C
- Sample Volume:100 μl
- Sensitivity:9.375 pg/ml
- Regulatory Status:RUO
- Cat. No.:76739-168
Specifications
About this item
Mouse Laminin gamma 3 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Laminin gamma 3 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay precision: Intra-assay: CV <8%, Inter-assay: CV <10%
Mouse Laminin gamma 3 ELISA kit (A247067) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Laminin gamma 3 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Laminin gamma 3 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Laminin gamma 3 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Laminin gamma 3 Antibody, which binds the captured Laminin gamma 3 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Laminin gamma 3 captured in each well. The concentration of Laminin gamma 3 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.