Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

• Simulated test samples
• Teaches immunodetection technique
• Hands-on dot blot analysis technique with necessary reagents and supplies

The method involves applying small volumes of antigens or test samples on protein binding membrane; the membrane captures and immobilizes proteins. The proteins immobilized on the membrane are probed with a specific antibody and a matching specific detection reagent. In this lab activity students screen test samples from a population and identify those who are carriers of a disease. Students learn how dot blotting applied in the real world, for example in clinical testing, forensic, and research laboratories.