NEBNext® Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 3)

About this item

NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 3) are built for more streamlined and informative NGS multiplexing, tracking the sequencing reaction at the individual molecule level and enabling bioinformatic removal of misassigned reads for optimal accuracy.

Index adaptors enable PCR-free library preparation
Superior and reliable ligation efficiency
More-sensitive detection of low-frequency single-nucleotide variants (SNVs)
Supports multiplexing for higher throughputs

These index adaptors contain a pair of unique dual indices (i5 and i7) and one UMI sequence, making the resulting library multiplex-ready, with or without PCR. This set includes 96 unique dual index UMI adaptors, packaged in a single-use 96-well plate with a pierceable foil seal.

The universal primer mix is supplied in a separate vial. Combining UMI Adaptors DNA Set 3 with one of the NEBNext DNA library prep kits is simple and straightforward if you follow the protocols found in the library prep kit manuals. With four sets of NEBNext Unique Dual Index UMI Adaptors available, you can multiplex up to 384 samples. Sample sheets for multiplexing are available.

Some Products May Appear Restricted

To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.

If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)

If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.