SARS-CoV-2 Recombinant (2019-nCoV) Spike S1+S2 (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) Protein (ECD, His Tag) (from Baculovirus-Insect Cells)
76642-110
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- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:Baculovirus (Insect cells)
- Species:SARS-CoV-2
- Size:100 µg
- Storage Conditions:–20…–80 °C
- Endotoxin Content:<1.0 EU per μg protein as determined by the LAL method
- Biological Activity:Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/ml (100 μl/well) can bind SARS-CoV-2 Spike S1+S2 (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) Protein (ECD, His Tag)(Cat:40589-V08B10), the EC50 of SARS-CoV-2 Spike S1+S2 (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) Protein (ECD, His Tag)(Cat:40589-V08B10) is 150-800 ng/ml.
- Reconstitution Instructions:A hardcopy of COA with reconstitution instruction is sent along with the products, please refer to it for detailed information
- Carrier-Free:Y
- Animal-Free:Y
- Protein Synonyms:ncov RBD|ncov s2|ncov spike|coronavirus s2|S|s1|Spike RBD|ncov s1|coronavirus spike|coronavirus s1|cov spike|RBD
- Protein/Peptide Name:Spike
- Purity:>90% as determined by SDS-PAGE
- Molecular Weight:The recombinant SARS-CoV-2 (2019-nCoV) Spike S1+S2 consists of 1209 amino acids and predicts a molecular mass of 134.5 kDa.
- Sequence:Met1-Pro1213(L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F)
- Formulation:Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10% glycerol, pH 8.0
- Shipping Temperature:Ambient
- Cat. No.:76642-110
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike S1+S2 (YP_009724390.1, with mutations L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) (Met1-Pro1213) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant P.1) which emerged in the Brazil.