Protein A is the most widely used affinity media for antibody purification.

Both Protein A and Protein G bind to the Fc region of IgG antibodies and with various selectivity to IgG subtypes. As a result, Protein A and protein G PhyTip columns can be used for purification of IgG antibodies, and IgG fragments that include the Fc region.

These columns have a demonstrated ability to extract functional IgG antibodies with very high purity from varying levels of background proteins, due to their high binding affinity and high capacity.

Columns come with validated dual flow chromatography protocols for purification of IgGs that can be transferred to any automated liquid handling system. Our buffer kits contain all the buffers is needed for the purification protocol. PhyTip columns purify samples by repeated back-and-forth cycles - a process called dual flow chromatography which allows complete binding interactions in each purification step.

Due to maximization of binding of the sample, less protein is lost during the purification process. The result is very high sample recoveries. The unique column design with a packed resin bed results in binding of the entire sample to a small resin bed volume. The purified sample can be eluted in small volumes, resulting in a highly concentrated sample.

Effective purification by PhyTip columns enables a full purification protocol of 96 samples in as little as 15 minutes. Protein A PhyTip columns comes with dual flow chromatography protocols that have been validated with real protein samples and can be transferred to any automated liquid handling system.