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SARS-CoV (Isolate Tor2) Recombinant Spike RBD Protein (His & AVI Tag), Biotinylated (from HEK293 Cells)
SARS-CoV (Isolate Tor2) Recombinant Spike RBD Protein (His & AVI Tag), Biotinylated (from HEK293 Cells)
  76630-992
 :  Sino Biological
 :  
SARS-CoV (Isolate Tor2) Recombinant Spike RBD Protein (His & AVI Tag), Biotinylated (from HEK293 Cells)
  76630-992
 :  Sino Biological
 :  40634-V27H-B-20
 :  

 

  • Conjugation:
    Biotin
  • Protein/Peptide Type:
    Recombinant
  • Source:
    HEK293 cells
  • Species:
    SARS
  • Size:
    20 µg
  • Storage Conditions:
    –20…–80 °C
  • Endotoxin Content:
    <1.0 EU per μg protein as determined by the LAL method.
  • Gene ID:
    NP_828851.1
  • Reconstitution Instructions:
    A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Carrier-Free:
    Y
  • Animal-Free:
    Y
  • Protein Synonyms:
    ncov RBD|ncov s2|ncov spike|coronavirus s2|S|s1|Spike RBD|ncov s1|coronavirus spike|coronavirus s1|cov spike|RBD
  • Protein/Peptide Name:
    Spike
  • Purity:
    >95% as determined by SDS-PAGE.
  • Molecular Weight:
    The recombinant SARS-CoV (isolate: Tor2) spike RBD consists of 249 amino acids and predicts a molecular mass of 28.4 kDa. As a result of glycosylation, it migrates as an approximately 37.1 kDa band in SDS-PAGE under reducing conditions.
  • Sequence:
    Arg306-Phe527
  • Shipping Temperature:
    Ambient
  • Cat. No.:
    76630-992

 

 

A DNA sequence encoding the SARS-CoV (isolate Tor2) spike RBD (NP_828851.1) (Arg306-Phe527) was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.