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SARS-CoV-2 (2019-nCoV) Recombinant Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (HPLC-verified) (from HEK293 Cells)
SARS-CoV-2 (2019-nCoV) Recombinant Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (HPLC-verified) (from HEK293 Cells)
Catalog # 76631-030
Supplier:  Sino Biological
CAS Number:  
SARS-CoV-2 (2019-nCoV) Recombinant Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (HPLC-verified) (from HEK293 Cells)
Catalog # 76631-030
Supplier:  Sino Biological
Supplier Number:  40589-V08H4-100
CAS Number:  

Specifications

  • Conjugation:
    Unconjugated
  • Protein/Peptide Type:
    Recombinant
  • Source:
    HEK293 cells
  • Species:
    SARS-CoV-2
  • Size:
    100 µg
  • Storage Conditions:
    –20…–80 °C
  • Endotoxin Content:
    <1.0 EU per μg of the protein as determined by the LAL method
  • Biological Activity:
    Measured by its binding ability in a functional ELISA.
    Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(10108-H05H) at 2 μg/ml (100 μl/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (40589-V08H4), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (40589-V08H4) is 10-50 ng/ml.
  • Gene ID:
    YP_009724390.1
  • Reconstitution Instructions:
    A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Carrier-Free:
    Y
  • Animal-Free:
    Y
  • Protein Synonyms:
    ncov RBD|ncov s2|ncov spike|coronavirus s2|S|s1|Spike RBD|ncov s1|coronavirus spike|coronavirus s1|cov spike|RBD
  • Protein/Peptide Name:
    Spike
  • Purity:
    >90% as determined by SDS-PAGE. >90% as determined by SEC-HPLC.
  • Molecular Weight:
    The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD(R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P), His tag) consists of 1248 amino acids and predicts a molecular mass of 138.5 kDa.
  • Sequence:
    Met1-Pro1213(R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)
  • Shipping Temperature:
    Ambient
  • Cat. No.:
    76631-030

Specifications

About this item

A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD) (YP_009724390.1) (Met1-Pro1213(R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)) was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.