HiTrap™ chromatography columns with MabSelect™ VL protein L resin. A redesigned protein L ligand captures antibody Fabs, bispecifics, and other variants containing a kappa light chain with high binding capacity and improved alkaline stability.

• High dynamic binding capacity for efficient capture of target molecules
• Stable when cleaned with 0.1 M NaOH, reducing risk for bioburden incidents
• Provides good resolution for product-related impurities in the capture of bispecific antibodies

As the diversity of antibody variants increases, so does the need for chromatography resins that can capture and purify these variants. As an alternative to protein A, protein L can be used to capture antibody Fabs, bispecific antibodies, and other molecules containing kappa variants of the variable light (VL) chain. Protein L binds human kappa light chain subtypes one, three, and four and mouse kappa light chain subtype 1.

MabSelect™ VL protein L resin is also available as bulk resins in different pack sizes, PreDictor™ RoboColumn® minicolumns, ReadyToProcess™ columns.