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Specifications
- Concentration:10000 units/ml
- Application:
- Source:
- Description:WarmStart Nt.BstNBI-1000 U
- Size:1000 units
- Environmentally Preferable:
- Cat. No.:76559-394
Specifications
About this item
WarmStart® Nt.BstNBI is a site specific nicking endonuclease cloned from Bacillus Stereothermophilus. Restriction Enzyme Cut Site: GAGTC(4/-5).
- WarmStart® functionality enables room temperature reaction setup
- Ideal for use in Strand Displacement Amplification (SDA)
- Nicking endonuclease in which only one strand of the duplex is cut ('nicked')
It is formulated with a reversibly-bound aptamer, which inhibits its nicking activity at temperatures below 40 °C. WarmStart Nt.BstNBI cleaves only one strand of DNA on a double-stranded DNA substrate. The nicking endonuclease catalyzes a single strand break four bases beyond the 3' side of the recognition sequence.