The NEBNext Ultra II DNA PCR-free Library Prep Kit contains the enzymes and buffers required to convert a broad range of pre-fragmented DNA input amounts into libraries for next-generation sequencing on the Illumina platform, all without an amplification step.

• Save time with an optimized PCR-free workflow, reduced hands-on time and automation compatibility
• Generate high quality libraries without an amplification step with inputs from 250 to 1000 ng
• Improved library uniformityFor use with NEBNext Unique Dual Index UMI Adaptors DNA Set 1
• Also compatible with other Illumina-compatible index adaptors with 5’ single T overhang

The fast, user-friendly workflow requires minimal hands-on time, and maintains excellent library yields and GC coverage without requiring PCR. Don’t be biased! The NEBNext® Ultra™ II DNA PCR-free Library Prep Kit for Illumina® can save you time and minimize amplification-induced sequence bias and errors with a complete solution for PCR-free NGS library preparation. Built on the robust and reliable Ultra II reagents and workflow from New England Biolabs®, you can trust that your samples are in the best hands – your own.