About this item
NEBExpress® E. coli Lysis Reagent is a chemical lysis solution composed of a proprietary mix of non-ionic and zwitterionic detergents and Tris-based buffer. It allows disruption of E. coli cells without denaturing soluble proteins.
- Scalable lysis reactions; compatible with high throughput workflows
- Suitable for extracting proteins, especially thermosensitive proteins
- Compatible with SDS-PAGE, Western blots, activity assays, immunoprecipitation, and purification
- Suitable for disrupting most Gram-negative bacterial cells
- Small (100 ml) and large (5×100 ml) suitable for lysing 20 to 100 g of cells, respectively
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