Mouse Recombinant R-Spondin 1/RSPO1 Protein (His Tag) (from CHO cells)

A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus.

Conjugation:
Unconjugated
Protein/Peptide Type:
Recombinant
Source:
CHO cells
Species:
Mouse
Storage Conditions:
–20…–80 °C
Endotoxin Content:
<1.0 EU per μg of the protein as determined by the LAL method
Biological Activity:
Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1 - 0.9 μg/ml in the presence of 5 ng/ml recombinant mouse Wnt3a.
Gene ID:
NP_619624.2
Reconstitution Instructions:
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Endotoxin-free:
N
Carrier-Free:
Y
Animal-Free:
Y
Protein Synonyms:
Rspondin|R-spondin
Protein/Peptide Name:
RSPO1
Purity:
>95% as determined by SDS-PAGE
Molecular Weight:
The secreted recombinant mouse RSPO1 comprises 256 amino acids with a predicted molecular mass of 28.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.
Sequence:
Met1-Gln265
Shipping Temperature:
Ambient