Mouse Recombinant IL27/Interleukin-27 Protein (His Tag) (from HEK293 Cells)

About this item

A DNA sequence encoding the mouse IL27 complex composed of EBI3 (O35228) (Met1-Pro228) and IL27A (Q8K3I6) (Phe29-Ser234) linked by an polypeptide linker was fused with the polyhistidine tag at the C-terminus.

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Specifications

Conjugation:
Unconjugated
Protein Function:
Cytokines and Growth Factors
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Mouse
Storage Conditions:
–20…–80 °C
Endotoxin Content:
<1.0 EU per μg of the protein as determined by the LAL method
Biological Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc (Cat:10489-H02H), The EC50 of human IL27RA-Fc (Cat:10489-H02H) is 0.26-0.62 μg/ml.
2. Measured in antiviral assay using HepG2 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 2-10ng/ml.
Reconstitution Instructions:
A hardcopy of COA with reconstitution instruction is sent along with the products, please refer to it for detailed information
Carrier-Free:
Y
Animal-Free:
Y
Protein Synonyms:
IL27p28|IL-27A|IL27A|IL-27|IL30|p28
Protein/Peptide Name:
IL27
Purity:
>85% as determined by SDS-PAGE
Molecular Weight:
The recombinant mouse IL27 comprises 442 amino acids and has a predicted molecular mass of 49.5 kDa. The apparent molecular mass of the protein is approximately 60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Sequence:
Met1-Pro228 & Phe29-Ser234
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping Temperature:
Ambient
Gene ID:
O35228 & Q8K3I6