Cynomolgus Recombinant CD32a/FCGR2A Protein (His & AVI Tag), Biotinylated (from HEK293 Cells)

A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32a (AAL92096.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

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Conjugation:
Biotin
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Cynomolgus monkey
Storage Conditions:
–20…–80 °C
Endotoxin Content:
<1.0 EU per μg of the protein as determined by the LAL method
Biological Activity:
Immobilized Cynomolgus CD32A Protein (His & AVI Tag), Biotinylated (Cat:90015-C27H-B) at 2 μg/ml (100 μl/well) can bind Human IgG2 Protein (257 Ser/Ala) (Cat:13504-HNAH), the EC50 is 15-140 ng/ml.
Gene ID:
AAL92096.1
Reconstitution Instructions:
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Carrier-Free:
Y
Animal-Free:
Y
Protein Synonyms:
CD32|FcGR|CD32A|IGFR2|Fc gamma RIIA|FCG2|FCGR2A1|CDw32|FCGR2
Protein/Peptide Name:
FCGR2A
Purity:
>95% as determined by SDS-PAGE
Molecular Weight:
The recombinant cynomolgus CD32a consists of 206 amino acids and has a calculated molecular mass of 22.9 kDa. The apparent molecular mass of the recombinant protein is approximately 33-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Sequence:
Met1-Ala220
Shipping Temperature:
Ambient