Rat Recombinant TNFR1/CD120a/TNFRSF1A Protein (Fc Tag) (from HEK293 Cells)
103666-414
:
- Conjugation:Unconjugated
- Protein Function:Immunotherapy
- Protein/Peptide Type:Recombinant
- Source:HEK293 cells
- Species:Rat
- Size:100 µg
- Storage Conditions:–20…–80 °C
- Endotoxin Content:<1.0 EU per μg of the protein as determined by the LAL method
- Biological Activity:1. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 ng/ml in the presence of 0.05 ng/ml of ratTNFα.
2. Immobilized mouse TNFa (80-235) (Cat:50349-MNAE ) at 10 μg/ml (100 μl/well) can bind rat TNFRSF1A-Fc, The EC50 of rat TNFRSF1A-Fc is 0.08-0.20 μg/ml. - Gene ID:P22934
- Reconstitution Instructions:A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
- Carrier-Free:Y
- Animal-Free:Y
- Protein Synonyms:TNFR1-d2|p55-R|TNF-R|TNF-R-I|TBP1|TNFR55|p55|CD120a|MS5|FPF|TNFAR|p60|TNFR1|TNFR60|TNF-R55
- Protein/Peptide Name:TNFRSF1A
- Purity:>90% as determined by SDS-PAGE.
- Molecular Weight:The recombinant rat TNFRSF1A/Fc is a disulfide-linked homodimer. The reduced monomer comprises 431 amino acids and has a predicted molecular mass of 47.9 kDa. The apparent molecular mass of the protein is approximately 59 kDa in SDS-PAGE under reducing conditions.
- Sequence:Met1-Ala211
- Shipping Temperature:Ambient
- Cat. No.:103666-414
A DNA sequence encoding the rat TNFRSF1A (P22934) (Met1-Ala211) was expressed, fused with the Fc region of human IgG1 at the C-terminus.