TOP-DNA and TOP-RNA enable good recovery of high-purity synthetic DNA and RNA oligonucleotides.

• Enhanced laboratory productivity, a complete solution for DNA and RNA oligonucleotide purification
• Pre-HPLC 'sample preparation' ability maximizes utility
• Freeing up operator time, TOP-DNA and TOP-RNA are high-throughput solutions that are automation friendly
• Reduces operating costs through lower reagent use
• Gravity or vacuum flow ensures flexibility

Agilent TOP-DNA and TOP-RNA remove interfering salts, incomplete synthesis products, and other impurities from synthetic DNA and RNA oligonucleotides in a few simple steps. TOP-DNA purifies oligonucleotides of up to 150-mer in length and from 200 nmol to 1 µmol. It can also be used for sample preparation before HPLC purification. TOP-RNA purifies both short and long RNA oligonucleotides, siRNA to 21-mer, and long RNA to 60-80-mer. The high-binding capacity purifies RNA oligonucleotides up to 1 µmol. The proprietary polymeric resin and validated protocol allow deprotection of 2'-hydroxyl group without removal of the 5'-trityl group.