Legacy zirconium-stabilized silica diol size exclusion columns (USP designation L35).

• High efficiency and reproducibility with short analysis time
• Compatible with organic modifiers and denaturants
• Semi-prep and prep column dimensions
• Wide usable pH range (3-8)

Agilent ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4000-900000 for globular proteins when using GF-250 and GF-450 columns in series.

The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8. The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions.

The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min. These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation.

Common applications include separations of protein monomers, dimers and aggregates, desalting, protein molecular weight estimation and separations of modified proteins. View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers.