VSV-Pseudovirus_SARS-CoV-2 with Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

Certificates

Supplier: ReVacc Scientific

About this item

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry the S protein of SARS-CoV-2 (GenBank: MN908947) (Wuhan strain or Wild type strain), with 18-aa cytoplasmic tail truncation for optimal infection.

Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. The titration result showing the undiluted pseudovirus generates signal ~10000-fold higher than uninfected control (cell alone as background). The neutralizing activity of two control mAbs was tested by using this pseudotyped virus.

As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Applications include Anti-SARS-CoV-2 neutralizing antibody screening at high throughput, Anti-SARS-CoV-2 drug screening at high throughput and SARS-CoV-2 pseudovirus transduction of target cells for viral entry and functional studies.

Packaging: Shipping with dry ice. Require −80 °C storage. Multiple freeze/Thaw cycles will reduce its sensitivity. Recommend only one cycle. Aliquot after the first thaw.

Caution: Handle it in biosafety cabinet in BSL-2. Contacted tips and tubes should be decontaminated by 10% disinfecting bleach. For research use only.

Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

• State issued document with your organization's Federal Tax ID Number
• State issued document with your organization's Resale Tax ID Number
• City or County issued Business License
• State Department of Health Services License
• Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.