Virus Recombinant Ebola Zaire Makona Trimeric GP (from Insect Cells)

A recombinant Ebola Zaire GPΔmuc protein (derived from strain Makona) consists of resides 32-309 and 461-628 (MLD residues 310-460 and transmembrane domain downstream of residue 628 deleted) and was produced by insect S2 cells, followed by purification.

Protein function: Bind to its receptor

This product contains C-terminal trimerization motif, enterokinase cleavage (DDDDK), a TwinStrep tag and 6 hexa-histidine tag.

This product has been run on SDS-PAGE with reduced condition. The binding to three published antibodies-KZ52, FVM04 and CA45 (Zhao, et al, Cell 2017, 169) was tested in ELISAs.

Conjugation:
Unconjugated
Protein/Peptide Type:
Recombinant
Source:
Insect cells
Species:
Virus
Storage Conditions:
–80 °C
Gene ID:
KR781609
Endotoxin-free:
N
Carrier-Free:
Y
Protease-free:
N
Animal-Free:
Y
Protein Synonyms:
Ebola trimeric GP Δmuc protein-Zaire Makona strain (His and Strep Tags)
Protein/Peptide Name:
Ebola zaire makona trimeric GP
Purity:
60 - 85%
Molecular Weight:
~ 60 kDa
Endotoxin Level:
None detected
Concentration:
0.2 mg/ml
Formulation:
Frozen liquid. In PBS pH 7.5
Nuclease-free:
N
Shipping Temperature:
Dry Ice
Tested Applications:
ELISA, SDS-PAGE