About this item
A recombinant trimeric form of the spike glycoprotein (S protein) of MERS-Cov (GenBank: JX869059.2) was produced by human embryonic kidney HEK293F cells, followed by purification.
- Protein function: Bind to its receptor
This product contains ectodomain of S protein with S1/S2 cleavage site knocked-down, and also had a pair of mutations (V1060P and L1061P), a HRV3C cleavage site, T4 foldon trimerization domain, C-terminal 8 hexa-histidine tag and twin-strep tag. Twin-strep tag can be used for streptavidin labeling.
This product has been run on SDS-PAGE with reduced condition and Native blue gels, with results proving trimerization and glycosylation. The binding to one MERS control mAb and published MERS27 antibody was tested in ELISAs.
Specifications
- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:Mammalian cells
- Species:Virus
- Storage Conditions:–80 °C
- Gene ID:JX869059
- Endotoxin-free:N
- Carrier-Free:Y
- Protease-free:N
- Animal-Free:Y
- Protein Synonyms:MERS-COV trimeric spike protein (His and Strep Tags)
- Protein/Peptide Name:MERS-COV trim spike protein
- Purity:90 - 100%
- Molecular Weight:~ 151 kDa
- Endotoxin Level:None detected
- Concentration:0.2 mg/ml
- Formulation:Frozen liquid. In PBS pH 7.5
- Nuclease-free:N
- Shipping Temperature:Dry Ice
- Tested Applications:ELISA, SDS-PAGE, NativeBlue
Specifications
Supplier: Kimberly-Clark
Supplier: Janitorial Supplies
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: VWR International
Supplier: Ansell Healthcare
Supplier: VWR International