The WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets.

• One-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
• Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the mix
• Includes LAMP Fluorescent Dye for real-time fluorescence detection
• WarmStart technology inhibits activity at room temperature

This kit includes the WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG), which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution, and a vial of LAMP Fluorescent Dye, an intercalating dye provided at 50X concentration that can be used to track a LAMP reaction in real-time by fluorescence measurement.

The inclusion of thermolabile UDG prevents carryover contamination, where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions.