Flow Cells for MinION™ and GridION™ Devices

Nanopore sequencing flow cells for use with MinION™ and GridION™ devices.

Flow cells contain the proprietary sensor array, Application-Specific Integrated Circuit (ASIC), and nanopores for DNA/RNA sequencing on MinION™ and GridION™ devices.

R10.4.1 flow cells contain a double reader-head and are suitable for experiments where high consensus accuracy is required. Compatible with Kit 14 chemistry, R10.4.1 flow cells generate data at a modal accuracy above 99%. R9.4.1 flow cells are compatible with the previous generation of nanopore sequencing chemistries. These flow cells and associated sequencing kits are anticipated to be discontinued in 2024.

RNA flow cells are designed to sequence native RNA with improved consensus accuracy and output. These flow cells are compatible with the latest generation of Direct RNA Sequencing Kit (77308-582).

Flow cells are available in single and multi-packs (8 pack, 48 pack, 96 pack) to suit sample throughput requirements. Flow cells in 48 and 96 packs can be split across multiple shipments of 8 flow cells. Please contact us to discuss specific requirements.

How flow cells work:
A user simply adds their prepared sequencing library to the flow cell and starts their sequencing experiment. Data is accumulated over time as DNA or RNA is sequenced by the nanopores. Individual read files are generated and are immediately available for analysis. As data is streamed in real time, a user can choose to stop a run as soon as sufficient data is gathered. A flow cell contains sufficient buffer to run for ~72 hours (under optimal conditions) and a user can choose to run continuously or run, stop, wash, and load a new sample until the buffer and nanopores are exhausted. Flow cells can be recycled through Oxford Nanopore Technologies.

: Flow cells in 48 and 96 packs can be scheduled across three deliveries (flow cells shipped in multiples of eight); must be delivered within nine months of purchase. Contact Oxford Nanopore Technologies to discuss requirements. Flow cells and kits are shipped together at 2 to 8 °C. The packaging is designed to protect the flow cells from freezing. R10.4.1 and R9.4.1 Flow Cells can be stored unopened at 2 to 8 °C for 12 weeks or unopened at room temperature for one month. RNA flow cells can be stored unopened at 2 to 8 °C for four weeks.

: Flow cells are shipped in a sealed pouch. This packaging also serves as the flow cell returns kit. Flow cell 8 packs are packaged in a single pouch containing all 8 flow cells. Users are requested to return their flow cells after use so that they can be responsibly recycled by Oxford Nanopore Technologies.

: Please carefully check flow cell and sequencing kit compatibility prior to ordering. Flow cell compatibility is provided on each individual sequencing kit page.