AutoScreen 96-well plate is used for purification of sequencing samples reactions prior to analysis on capillary array electrophoresis (CAE) sequencers such as MegaBACE 1000 or ABI Prism sequencing instruments.

• Includes DNA Grade Sephadex G-50 rehydrated in double-distilled water as well as 0.05% Kathon™ CG/ICP Biocide added as a preservative
• Preferred loading volume is 15 to 20 µl. The suggested range for loading volumes to achieve optimum purification is 10 to 20 µl
• A plate lid may be used during this centrifugation step to improve consistency of volume recovery and reduce the likelihood of contamination during purification steps in sample sequencing

AutoScreen 96-Well Plates used with MegaBACE 1000 can show two to three times lower signal intensities compared with intensities from ethanol-precipitated reactions. This does not affect read lengths. AutoScreen 96 is also applicable to other size exclusion chromatography applications.