NEBExpress Ni-NTA Magnetic Beads, New England Biolabs

About this item

An affinity matrix for the small-scale isolation and purification of polyhistidine-tagged (His-tagged) fusion proteins in manual or automated formats. Immobilized Metal Affinity Chromatography (IMAC) purifications employing Ni-NTA (nickel-nitrilotriacetic acid) magnetic beads can be performed under native or denaturing conditions which permit efficient binding and purification of insoluble proteins, proteins that aggregate in inclusion bodies, or proteins that possess a tertiary structure that sequester the polyhistidine affinity tag.

NTA coordination exhibits low Nickel ion leaching
Tolerates a wide range of conditions, including the presence of protein denaturants and detergents
Low non-specific binding permits immobilized fusion proteins to be used in subsequent experiments

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