Q5U Hot Start high-fidelity DNA Polymerase is a modified version of Q5 high-fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3' to 5' exonuclease activity, and is fused to a processivity-enhancing Sso7d domain.

• Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (FFPE)
• Prevent carryover contamination when combined with dUTP and Uracil DNA Glycosylase (UDG) treatment
• Generate higher assembly efficiency and improve accuracy in USER cloning
• Enable room temperature setup with aptamer-based hot start formulation

Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.