Quick CIP is a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5 and 3 ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- and deoxyribonucleoside triphosphates (NTPs and dNTPs).

• Rapid and irreversible heat inactivation eliminates unwanted activity
• Improved storage stability versus native enzyme
• Faster reaction setup (no supplemental additives like zinc required) and shorter incubation time
• Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)

Quick CIP is useful in many applications that require the dephosphorylation of DNA or RNA ends. In cloning, dephosphorylation prevents re-ligation of linearized plasmid DNA. The enzyme can quickly dephosphorylate 5 protruding, 5 recessed, and blunt ends in just 10 minutes. Quick CIP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis.