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Specifications
- Source:
- Species:
- Size:250 units
- Environmentally Preferable:
- Enzyme Name:Thermostable RNase H
- Concentration:5000 units/ml
- Cat. No.:76284-680
Specifications
About this item
Thermostable RNase H is an endoribonuclease that is functional at high temperatures and selectively hydrolyzes the phosphodiester bonds of an RNA strand in an RNA:DNA hybrid molecule, while leaving the DNA strand intact.
- Recombinant enzyme supplied with 10X Reaction Buffer
- Does not digest single or double-stranded DNA
- Active and stable above 37 °C over a broad temperature range
- Requires magnesium chloride for activityInactivated with proteinase K treatment or addition of excess EDTA
In addition, Thermostable RNase H does not degrade single- or double-stranded RNA or DNA. While the Thermostable and E. coli RNase H homologs both exhibit these same endoribonucleolytic properties, the thermostable enzyme displays activity at higher temperatures with optimal activity above 65 °C. These properties allow use in applications where higher assay temperatures are desired.