AffinityScript cDNA Synthesis Kits, Agilent Technologies
About this item
Provides the highest efficiency of conversion of RNA to cDNA, fully optimized for qPCR applications.
- Optimized for real-time quantitative PCR (qPCR) application;Produces cDNA in 15 min with linear detection from 3 pg to 3 µg total RNA
- Creates up to 12 kb cDNA in 25 min or less
- Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format
The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.
The streamlined protocol produces cDNA in 15 min with linear detection from 3 pg to 3 µg of total RNA. The easy-to-use process generates up to 12 kb cDNA in 25 min or less. Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format, using SYBR Green or probe-based detection.
The AffinityScript Multiple Temperature cDNA synthesis kit contains Agilent’s popular, highly thermostable, qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase for robust yields, from 37 to 60 °C, with nearly full activity between 45 to 55 °C. With the AffinityScript multiple temperature cDNA synthesis kit, Agilent offers enzymes with a broad thermal profile.
The Agilent AffinityScript Multiple Temperature Reverse Transcriptase (RT) system is optimized to produce high cDNA yields over the broadest temperature range. During analysis the multi-temp RT device also ensures high affinity to primer-template complexes. cDNA can be synthesized in amounts up to 20 kb.
The system’s multi-temperature capability allows you to change RT reaction temperature without having to change the reverse transcriptase, for a faster and more efficient analysis. In addition, when amplifying low RNA input amounts and resolving RNA secondary structures it delivers high cDNA yields, and can operate effectively at temperatures up to 55 °C.
Caution: For research use only. Not for use in diagnostic procedures.
5 Options Available Below
Product Details & Documents
Provides the highest efficiency of conversion of RNA to cDNA, fully optimized for qPCR applications.
- Optimized for real-time quantitative PCR (qPCR) application;Produces cDNA in 15 min with linear detection from 3 pg to 3 µg total RNA
- Creates up to 12 kb cDNA in 25 min or less
- Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format
The AffinityScript qPCR cDNA Synthesis Kit offers efficient conversion of RNA to cDNA. Fully optimized for real-time quantitative PCR (qPCR) applications, the kit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.
The streamlined protocol produces cDNA in 15 min with linear detection from 3 pg to 3 µg of total RNA. The easy-to-use process generates up to 12 kb cDNA in 25 min or less. Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format, using SYBR Green or probe-based detection.
The AffinityScript Multiple Temperature cDNA synthesis kit contains Agilent’s popular, highly thermostable, qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase for robust yields, from 37 to 60 °C, with nearly full activity between 45 to 55 °C. With the AffinityScript multiple temperature cDNA synthesis kit, Agilent offers enzymes with a broad thermal profile.
The Agilent AffinityScript Multiple Temperature Reverse Transcriptase (RT) system is optimized to produce high cDNA yields over the broadest temperature range. During analysis the multi-temp RT device also ensures high affinity to primer-template complexes. cDNA can be synthesized in amounts up to 20 kb.
The system’s multi-temperature capability allows you to change RT reaction temperature without having to change the reverse transcriptase, for a faster and more efficient analysis. In addition, when amplifying low RNA input amounts and resolving RNA secondary structures it delivers high cDNA yields, and can operate effectively at temperatures up to 55 °C.
Caution: For research use only. Not for use in diagnostic procedures.
Product Family Options
Product Information
- DetailsNo. of reactionsAvailabilityPrice
- ST600559AffinityScript qPCR cDNA synthesis KitAffinityScript qPCR cDNA synthesis KitKit delivers fast, efficient cDNA synthesis for qRT-PCR. Produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg total RNA. Creates up to 12 kb cDNA in 25 min or less50Log in for availabilityEach$450.73Supplier Number: 600559
- ST200436AffinityScript cDNA Synthesis KitAffinityScript cDNA Synthesis KitKit utilizes our qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.50Log in for availabilityEach$407.54Supplier Number: 200436
- ST600107AffinityScript multiple temperature reverse transcriptaseAffinityScript multiple temperature reverse transcriptaseEngineered to be highly thermostable, allowing you to reverse transcribe at your preferred reaction temperature. Achieve a robust cDNA yield with even GC-rich sequences. Increased activity at all temperatures in a broad operating range, from 37 to 55 °C. The system is able to synthesize cDNA up to 20 kb50Log in for availabilityEach$284.61Supplier Number: 600107
- 99902-652AffinityScript multiple temperature reverse transcriptaseAffinityScript multiple temperature reverse transcriptaseEngineered to be highly thermostable, allowing you to reverse transcribe at your preferred reaction temperature. Achieve a robust cDNA yield with even GC-rich sequences. Increased activity at all temperatures in a broad operating range, from 37 to 55 °C. The system is able to synthesize cDNA up to 20 kb200Log in for availabilityEach$1,017.75Supplier Number: 600109
- 97066-440AffinityScript one-step RT-PCR KitAffinityScript one-step RT-PCR KitReduced PCR cycling times and a one-tube, master mix format. Combined performance of Herculase II fusion DNA polymerase and the ArchaeMaxx PCR enhancing factor, delivering superior PCR yield and reliability. Robust performance for difficult and GC-rich targets. Optimized for target lengths up to 9.5 kb.100Log in for availabilityKit of 2$637.89Supplier Number: 600188Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
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