NEBNext® Multiplex Oligos for Illumina are an essential piece of the NEBNext® suite of library preparation products, available in several different configurations for use with NEBNext® products or other standard Illumina-compatible library preparation protocols.

• Index adaptors enable PCR-free library preparation
• More-sensitive detection of low-frequency single-nucleotide variants (SNVs)
• Supports multiplexing for higher throughputs

This set was designed and optimized for use in DNA sequencing workflows, including PCR-free. These unique dual index adaptors also include a unique molecular identifier (UMI) sequence to enable the identification and removal of PCR errors or duplicates from amplified libraries. Supplied as 96 unique, pre-annealed index adaptors with UMIs, packaged in a single-use 96-well plate with a pierceable foil seal. Universal primers are included for applications requiring PCR amplification.