Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  Fc Tag (active trimer) (MALS verified)
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  FACS analysis shows that Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5269) can bind to 293T-CD40 cells surface CD40. The concentration of Human CD40 Ligand is 0.3 μg/mL (Routinely tested). Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5269) stimulates secretion of IL-6 by human B cells. The ED50 for this effect is 9.68-11.48 ng/mL in the presence of 5 ng/mL of Recombinant Human IL‑4 (Routinely tested).
• Gene ID:
  P29965-1
• Protein Synonyms:
  TRAP|CD40L|IMD3|gp39|CD154|CD40LG|IGM|T-BAM|TNFSF5|HIGM1
• Protein/Peptide Name:
  CD40 Ligand
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular Weight:
  74.7 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT