Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  active trimer (MALS verified)
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Human BAFFR Protein, Fc Tag (Cat. No. BAR-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human BAFF Protein, His,Flag Tag (Cat. No. BAF-H52D4) with an affinity constant of 5.54 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Human BAFF, His,Flag Tag, active trimer (Cat. No. BAF-H52D4) stimulates proliferation of mouse spleen cells. The EC50 for this effect is 1.97-2.90 ng/mL (Routinely tested).
• Protein Synonyms:
  CD257|ZTNF4|THANK|TALL-1|TNFSF20|DTL|TNFSF13B|TALL1|BLYS|BAFF
• Protein/Peptide Name:
  BAFF
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular Weight:
  52.5 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH 6.0. Normally Trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT