Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  SARS-CoV-2
• Tag sequence:
  Mouse IgG2a Fc Tag
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Loaded SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) on AMC Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 3.95 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). FACS analysis shows that SARS-CoV-2 S1 protein, Mouse IgG2a Fc Tag (Cat. No. S1N-C5257) can bind to Vero E6 cells surface ACE2. The concentration of SARS-CoV-2 S1 protein is 3 μg/ml. (Routinely tested).
• Protein Synonyms:
  Spike|Spike glycoprotein Subunit1|S glycoprotein Subunit1|S1 protein|Spike protein S1|COVID-19
• Protein/Peptide Name:
  S1 protein
• Purity:
  >90% as determined by SDS-PAGE.
• Molecular Weight:
  101.9 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT