Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  SARS-CoV-2
• Tag sequence:
  Mouse IgG2a Fc Tag (MALS verified)
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Loaded SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat. No. SPD-C5259) on AMC Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 3.44 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). FACS analysis shows that the binding of SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat. No. SPD-C5259) to Vero E6 cells surface ACE2 was inhibited by increasing concentration of Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35). The concentration of SARS-CoV-2 S protein RBD used is 5μg/ml. The IC50 is 10.33 μg/ml (Routinely tested).
• Protein Synonyms:
  Spike|S glycoprotein RBD|Spike glycoprotein Receptor-binding domain|Spike protein RBD|S protein RBD|COVID-19
• Protein/Peptide Name:
  S protein RBD
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular Weight:
  52.0 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS,pH7.4 . Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT