Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  SARS-CoV-2
• Tag sequence:
  His Tag (MALS verified)
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 17 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Human ACE2, Fc Tag (Cat. No. AC2-H5257) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 34.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Biotinylated Human Neuropilin-1, His,Avitag (Cat. No. NR1-H82E3) on SA Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 1.01 μM as determined in BLI assay (ForteBio Octet Red96e);;;;;
• Protein Synonyms:
  Spike|S glycoprotein RBD|Spike glycoprotein Receptor-binding domain|Spike protein RBD|S protein RBD|COVID-19
• Protein/Peptide Name:
  S protein RBD
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular Weight:
  26.5 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT