Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Rhesus monkey
• Tag sequence:
  His Tag (SPR & BLI verified)
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Rhesus macaque CD32a, His Tag (SPR & BLI verified) (Cat. No. CDA-R52H4) captured on CM5 Chip via anti-His antibody can bind Rituximab with an affinity constant of 3.89 μM as determined in SPR assay (Biacore T200) (QC tested). Loaded Rhesus macaque CD32a, His Tag (SPR & BLI verified) (Cat. No. CDA-R52H4) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 3.3 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Rituximab on FAB2G Biosensor, can bind Rhesus macaque CD32a, His Tag (SPR & BLI verified) (Cat. No. CDA-R52H4) with an affinity constant of 5.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Rituximab on Protein A Biosensor, can bind Rhesus macaque CD32a, His Tag (SPR & BLI verified) (Cat. No. CDA-R52H4) with an affinity constant of 7.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  H9BMP0-1
• Protein Synonyms:
  CD32|CD32a|IGFR2|FCGR2A1|FCG2 |FCGR2A
• Protein/Peptide Name:
  Fc gamma RIIA/ CD32a
• Purity:
  >90% as determined by SDS-PAGE.
• Molecular Weight:
  22.3 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT