Unconjugated

Recombinant

Human

100 µg

His Tag (MALS verified)

–20 °C

Less than 1.0 EU per μg by the LAL method.

Anti-Human TIGIT MAb (Mouse IgG1) captured on CM5 chip via Anti-Mouse antibodies surface, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 3.93 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Anti-Human TIGIT MAb (Mouse IgG1) on AMC Biosensor, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 3.72 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Human CD155, Fc Tag (Cat. No. CD5-H5251) on Protein A Biosensor, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 0.23 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) on Protein A Biosensor, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 0.732 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). FACS assay shows that Human TIGIT, His Tag (Cat. No. TIT-H52H3) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.3 μg/mL (Routinely tested).

Q495A1-1

VSTM3|TIGIT|VSIG9

TIGIT

>95% as determined by SDS-PAGE.

14.9 kDa

Low

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

RT

103815-518