Specifications

• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Species:
  Human
• Size:
  100 µg
• Tag sequence:
  Fc Tag (MALS verified)
• Storage Conditions:
  –20 °C
• Endotoxin Content:
  Less than 1.0 EU per μg by the LAL method.
• Biological Activity:
  Measured by its binding ability in a functional ELISA. Immobilized Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) at 5 μg/mL (100 μL/well) can bind Human TIGIT, Fc Tag (Cat. No. TIT-H5254) with a linear range of 0.6-5 ng/mL (QC tested). Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT, Fc Tag (Cat. No. TIT-H5254) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) with a linear range of 0.313-10 μg/mL (Routinely tested). Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested). Loaded Human TIGIT, Fc Tag (Cat. No. TIT-H5254) on Protein A Biosensor, can bind Human CD155, His Tag (Cat. No. CD5-H5223) with an affinity constant of 0.98 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). FACS assay shows that recombinant Human TIGIT, Fc Tag (Cat. No. TIT-H5254) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.1 μg/mL (Routinely tested).
• Gene ID:
  Q495A1-1
• Protein Synonyms:
  VSTM3|TIGIT|VSIG9
• Protein/Peptide Name:
  TIGIT
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular Weight:
  39.7 kDa
• Endotoxin Level:
  Low
• Formulation:
  Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 10 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  RT
• Cat. No.:
  103815-460